1 M MOPS pH 7.0
RNA gels (10× MOPS), bacterial minimal media.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| MOPS (free acid) | 20.93 g | 104.6 g | 209.3 g |
| NaOH (10 M)approximate; finish with a pH meter | 3.869 mL | 19.34 mL | 38.69 mL |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve MOPS (free acid) in about 80 % of the final volume of purified water.
- Adjust to pH 7.0 at 25 °C with NaOH (10 M), adding slowly while stirring.
- Make up to the final volume with water.
- Filter-sterilise (0.22 µm) and store at 4 °C.