Buffer recipes
Masses and titrant volumes for common buffers at 25 °C. Open any recipe in the calculator to change concentration, pH, volume or temperature.
TrispKa 8.07
- 1 M Tris-HCl pH 8.0Stock for TE
- 1 M Tris-HCl pH 7.5General protein and nucleic acid buffers
- 1 M Tris-HCl pH 7.4Physiological-pH buffers
- 1.5 M Tris-HCl pH 8.8Resolving gel buffer for SDS-PAGE (Laemmli)
- 1 M Tris-HCl pH 6.8Stacking gel and sample buffer for SDS-PAGE
- 50 mM Tris-HCl pH 7.5Working buffer for enzyme assays and protein storage