Buffer recipes
Standard preparations at 25 °C. Open a recipe to adjust concentration, pH, or volume.
TrispKa 8.07
- 1 M Tris-HCl pH 8.0Stock for TE
- 1 M Tris-HCl pH 7.5General protein and nucleic acid buffers
- 1 M Tris-HCl pH 7.4Physiological-pH buffers
- 1.5 M Tris-HCl pH 8.8Resolving gel buffer for SDS-PAGE (Laemmli)
- 1 M Tris-HCl pH 6.8Stacking gel and sample buffer for SDS-PAGE
- 50 mM Tris-HCl pH 7.5Working buffer for enzyme assays and protein storage