100 mM glycine-HCl pH 2.5
Elution of antibodies from protein A/G columns.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| Glycine | 0.7507 g | 3.754 g | 7.507 g |
| HCl (1 M)approximate; finish with a pH meter | 4.145 mL | 20.73 mL | 41.45 mL |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve Glycine in about 80 % of the final volume of purified water.
- Adjust to pH 2.5 at 25 °C with HCl (1 M), adding slowly while stirring.
- Make up to the final volume with water.
- Filter-sterilise (0.22 µm) and store at 4 °C.