500 mM MES pH 6.0
Plant tissue culture, EDC/NHS coupling, cation-exchange chromatography.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| MES (free acid) | 9.762 g | 48.81 g | 97.62 g |
| NaOH (10 M)approximate; finish with a pH meter | 2.213 mL | 11.07 mL | 22.13 mL |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve MES (free acid) in about 80 % of the final volume of purified water.
- Adjust to pH 6.0 at 25 °C with NaOH (10 M), adding slowly while stirring.
- Make up to the final volume with water.
- Filter-sterilise (0.22 µm) and store at 4 °C.