500 mM PIPES pH 6.8
Microtubule and cytoskeleton buffers (PEM, BRB80). PIPES free acid dissolves only as the pH rises; add NaOH while stirring.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| PIPES (free acid) | 15.12 g | 75.59 g | 151.2 g |
| NaOH (10 M)approximate; finish with a pH meter | 2.615 mL | 13.08 mL | 26.15 mL |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve PIPES (free acid) in about 80 % of the final volume of purified water.
- Adjust to pH 6.8 at 25 °C with NaOH (10 M), adding slowly while stirring.
- Make up to the final volume with water.
- Filter-sterilise (0.22 µm) and store at 4 °C.