100 mM carbonate-bicarbonate pH 9.6
Coating buffer for ELISA plates. Use within a week; absorbs CO₂ from air.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| NaHCO₃ | 0.7082 g | 3.541 g | 7.082 g |
| Na₂CO₃ (anhydrous) | 0.1664 g | 0.8319 g | 1.664 g |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve NaHCO₃ in about 80 % of the final volume of purified water.
- Check the pH; it should read 9.6 at 25 °C. Adjust with dilute acid or base only if needed.
- Make up to the final volume with water.
- Use fresh or store at 4 °C for up to a month.