100 mM sodium bicarbonate pH 8.3
NHS-ester labelling of proteins and antibodies.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| NaHCO₃ | 0.8323 g | 4.162 g | 8.323 g |
| Na₂CO₃ (anhydrous) | 0.0098 g | 0.049 g | 0.098 g |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve NaHCO₃ in about 80 % of the final volume of purified water.
- Check the pH; it should read 8.3 at 25 °C. Adjust with dilute acid or base only if needed.
- Make up to the final volume with water.
- Filter-sterilise (0.22 µm) and store at 4 °C.
- Target pH is more than 1 unit from pKa (6.35); buffer capacity will be low.