100 mM sodium acetate pH 5.0
Glycosidase and acid phosphatase assays.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| Acetic acid (glacial, 99.7 %) | 0.2095 mL | 1.048 mL | 2.095 mL |
| Sodium acetate (anhydrous) | 0.5207 g | 2.603 g | 5.207 g |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve Acetic acid (glacial, 99.7 %) in about 80 % of the final volume of purified water.
- Check the pH; it should read 5.0 at 25 °C. Adjust with dilute acid or base only if needed.
- Make up to the final volume with water.
- Autoclave or filter-sterilise and store at room temperature.