20 mM HEPES pH 7.4
Working buffer for live-cell imaging and electrophysiology.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| HEPES (free acid) | 0.4766 g | 2.383 g | 4.766 g |
| NaOH (1 M)approximate; finish with a pH meter | 0.9082 mL | 4.541 mL | 9.082 mL |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve HEPES (free acid) in about 80 % of the final volume of purified water.
- Adjust to pH 7.4 at 25 °C with NaOH (1 M), adding slowly while stirring.
- Make up to the final volume with water.
- Filter-sterilise (0.22 µm) and store at 4 °C.