1.5 M Tris-HCl pH 8.8
Resolving gel buffer for SDS-PAGE (Laemmli).
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| Tris base | 18.17 g | 90.86 g | 181.7 g |
| HCl (12 M)approximate; finish with a pH meter | 1.962 mL | 9.811 mL | 19.62 mL |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve Tris base in about 80 % of the final volume of purified water.
- Adjust to pH 8.8 at 25 °C with HCl (12 M), adding slowly while stirring.
- Make up to the final volume with water.
- Use fresh or store at 4 °C for up to a month.