1 M Tris-HCl pH 7.5
General protein and nucleic acid buffers, TBS stock.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| Tris base | 12.11 g | 60.57 g | 121.1 g |
| HCl (12 M)approximate; finish with a pH meter | 6.566 mL | 32.83 mL | 65.66 mL |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve Tris base in about 80 % of the final volume of purified water.
- Adjust to pH 7.5 at 25 °C with HCl (12 M), adding slowly while stirring.
- Make up to the final volume with water.
- Autoclave or filter-sterilise and store at room temperature.