1 M potassium phosphate pH 7.0
Stock for enzyme and mitochondrial buffers.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| KH₂PO₄ | 8.344 g | 41.72 g | 83.44 g |
| K₂HPO₄ | 6.738 g | 33.69 g | 67.38 g |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve KH₂PO₄ in about 80 % of the final volume of purified water.
- Check the pH; it should read 7.0 at 25 °C. Adjust with dilute acid or base only if needed.
- Make up to the final volume with water.
- Autoclave or filter-sterilise and store at room temperature.