100 mM sodium phosphate pH 6.5
Peroxidase and phosphatase assays.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| NaH₂PO₄ (anhydrous) | 1 g | 5.001 g | 10 g |
| Na₂HPO₄ (anhydrous) | 0.2361 g | 1.181 g | 2.361 g |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve NaH₂PO₄ (anhydrous) in about 80 % of the final volume of purified water.
- Check the pH; it should read 6.5 at 25 °C. Adjust with dilute acid or base only if needed.
- Make up to the final volume with water.
- Autoclave or filter-sterilise and store at room temperature.