100 mM sodium phosphate pH 7.4
Fixation, immunostaining and general biochemistry. Made by mixing monobasic and dibasic salts; no titration needed.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| NaH₂PO₄ (anhydrous) | 0.4642 g | 2.321 g | 4.642 g |
| Na₂HPO₄ (anhydrous) | 0.8704 g | 4.352 g | 8.704 g |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve NaH₂PO₄ (anhydrous) in about 80 % of the final volume of purified water.
- Check the pH; it should read 7.4 at 25 °C. Adjust with dilute acid or base only if needed.
- Make up to the final volume with water.
- Autoclave or filter-sterilise and store at room temperature.