100 mM sodium phosphate pH 7.0
Enzyme assays and spectroscopy.
| Component | 100 mL | 500 mL | 1 L |
|---|---|---|---|
| NaH₂PO₄ (anhydrous) | 0.7356 g | 3.678 g | 7.356 g |
| Na₂HPO₄ (anhydrous) | 0.5492 g | 2.746 g | 5.492 g |
| Final volume with water | 100 mL | 500 mL | 1 L |
- Dissolve NaH₂PO₄ (anhydrous) in about 80 % of the final volume of purified water.
- Check the pH; it should read 7.0 at 25 °C. Adjust with dilute acid or base only if needed.
- Make up to the final volume with water.
- Autoclave or filter-sterilise and store at room temperature.